This guide is a ‘how-to’ guide and proof of concept, i.e., (i) how to formulate the policy scenarios that TEEB Agrifood is applied to, (ii) what data, methods and disciplinary expertise are required, (iii) what is the sequencing, (iv) what challenges apply to assessing policy options with TCA and how might they be addressed,(v) how are results mainstreamed into policy making, and(vi) what was the measurable impact of UNEP TEEB AgriFood applications?