The Guidance Manual for TEEB Country Studies provides both technical and operational guidance on how countries may conduct a TEEB Country Study. It outlines the various steps that may be taken to initiate and implement a country study, communicate its findings, and implement the recommendations of the study. The target audience of this manual is researchers and decision-makers at the national or sub-national level considering or currently undertaking a TEEB country study (TCS). Its purpose is to provide guidance throughout the entire TCS cycle i.e., from initiation, to policy analysis and ecosystem service valuations, communicating findings, following up and finally applying results. The manual is not intended to be prescriptive but rather provides directions and guidelines for TEEB implementation, which must be adapted according to the specific circumstances and objectives of the country. It also outlines the quality and completeness criteria for such a study to become formally endorsed as a “TEEB Country Study” by the TEEB Advisory Board. The manual was developed by the Helmholtz Centre for Environmental Research (UFZ), UNEP, Deutsche Gesellschaft fuer Internationale Zusammenarbeit (GIZ) and the Institute for European Environmental Policy (IEEP) and was launched during the Trondheim Conference on Biodiversity on 28 May 2013.