This Strategic Framework: i) Presents the background and context for the collaboration between the Food and Agriculture Organization of the United Nations (FAO), the World Organisation for Animal Health (OIE) and the World Health Organization (WHO) (“the Tripartite”) with the United Nations Environment Programme (UNEP) on antimicrobial resistance (AMR); ii) Describes the comparative advantage and catalytic role of the four organizations in the One Health response to AMR in support of efforts by their Members, civil society, the private sector and other stakeholders; and iii) Presents a theory of change including the goals, objectives, desired impact at country level, intermediate outcomes and related Tripartite and UNEP functions. The Strategic Framework is intended to reflect the joint work of the four organizations to advance a One Health response to AMR.